PUREBlock^TM is a ready-to use blocking solution for western blots. The highly efficient blocking enables blots with significantly reduced background levels. Primary and secondary antibodies can be directly diluted within PUREBlock^TM. PUREBlock^TM is applied as any other blocker for immunodetection on western blots. Existing protocols or incubation times do not have to be changed, at least as a starting point.

PUREBlock^TM contains a selected mixture of casein fragments together with other blocking components. Compared to milk powder or BSA, we can regularly expect improved results. Moreover, PUREBlock^TM offers enhanced binding conditions of the antibodies, compared to other blocking buffers.

Due to reduced cross-reactivity, depending on the individual experimental conditions, lower antibody concentrations may be required without loosing signal intensity.

PUREBlock^TM is one of the active components of the PURITY^TM HRP reagent. It is suited for everybody who wants to improve the results of a classical sequencial western protocol. Including those, whose antibodies do not have the affinity, that is required for Western Blot 2.0 with PURITY^TM.

• ready to use solution
• no need to exchange current protocols
• defined components ensure consistent high quality
• highly efficient blocking of unspecified binding sites
• reduced background development
• enhanced reproducibility
• less cross-reactivity
• replaces BSA or milk powder
• replaces TBST when using phosphospecific antibodies