Western-Blot 2.0 – sensitive, specific, reliable, fast


 

 

Since decades, western blots are carried out according to the more or less same sequential protocol.

The typical experimental workflow starting with the electrophoresis until the detection usually takes two full working days. Frequently it requires a laborious and time-consuming optimization of numerous individual steps. Many parameters have to be adjusted to limit undesired phenomena (background, non-specific bands , artifacts) until the final results are in a presentable, meaningful and quantifiable form.

 

The new PURITY™ WB HRP Detection System virtually eliminates the need for optimizations. Another unique feature of Western blot 2.0: the entire immune reaction is limited to one single step. Thus, electrophoresis , blotting, detection, evaluation and planning of the following experiment can be performed during one working day. This saves time and resources.

 

PURITY™ contains all relevant components needed for the reaction - except for your specific primary antibody. Its special formulation allows PURITY™ to block nonspecific binding sites on the membrane and to create reaction conditions, that prevent low-affinity (nonspecific ) binding of the antibody. In addition, PURITY™ significantly speeds up and simplifies the total western workflow dramatically.

 

The perfect complement to PURITY™ is the new chemiluminescent substrate PURECL™. It refutes the common observation that CL sensitive detection are often realized only at the price of an increased background.

 

PURECL™ does both - it is highly sensitive and at the same time largely neutral to background. PURITY™ and PURECL™ pair ideally and follow the working principles of our FUSION imagers.

 

Highest sensitivity and lowest background noise!

 

 

Keywords : Western Blot , background , sensitivity, chemiluminescent substrate

 

 

 

Art No

PU

Reagent

Amount

PU4300500

Kit

PURITYTM Anti Rabbit

500 ml

PU4300100

Kit

PURITYTM Anti Rabbit

100 ml

PU4300020

Kit

PURITYTM Anti Rabbit

20 ml

PU4200500

Kit

PURITYTM Anti Mouse

500 ml

PU4200100

Kit

PURITYTM Anti Mouse

100 ml

PU4200020

Kit

PURITYTM Anti Mouse

20 ml

PU4100500

Kit

PURECLTM Western Substrate

1000 ml

PU4100100

Kit

PURECLTM Western Substrate

200 ml

PU4100020

Kit

PURECLTM Western Substrate

40 ml